England manager Gareth Southgate will be without influential midfielder Declan Rice for Thursday's World Cup Qualifying encounter against Albania.
The West Ham star was named in the initial squad but hasn't trained after falling ill.
Mason Mount and Luke Shaw are in the squad, but are yet to train as the former is recovering from dental surgery, while the latter suffered concussion in Manchester United's loss against Manchester City.
At the moment, there are no plans for any of the above trio to be replaced.
One player who has been called up as a replacement is Emile Smith Rowe, who has earned his first place in a senior England squad, with Marcus Rashford dropping out.
England are unbeaten in Group I so far and sit top of the standings with 20 points taken from their opening eight fixtures.
Jordan Pickford will start in goal in behind a likely back four of Kyle Walker, Harry Maguire, John Stones and Ben Chilwell.
With Rice out, it should be an easy decision for Southgate to partner Jordan Henderson in midfield with Kalvin Phillips.
Raheem Sterling, Phil Foden and Jack Grealish will likely be the favoured midfield three in behind lone striker Harry Kane.