The club confirmed six - Heorhiy Tsytaishvili, Denys Harmash, Mikkel Duelund, Olexandr Karavayev, Tudor Baluta, and Mykola Shaparenko – had tested positive for coronavirus ahead of the trip to Spain, as well as assistant coach Emil Caras and four other members of staff.
This comes as three players had tested positive last week, although the side were able to maintain their unbeaten start to the league campaign with a 2-1 win over Dnipro.
Two of the positive cases are goalkeepers which resulted in 18-year-old Ruslan Neshcheret making his senior debut for the club, and he looks set to play in goal again on Tuesday night.
On top of those cases, captain Serhiy Sydorchuk is suspended for the clash while three other first-teamers are recovering from injuries and are not expected to take part.
Dynamo will be aiming to emulate their rivals Shakhtar Donetsk who defeated Real Madrid last month with 10 players and nine coaches absent with COVID-19.